This study was carried out to observe the effect of nutritional condition on the change of protein metabolism in the animal body by feeding on imbalanced protein diet. A total 242 growing male albino rats, weighing 115^120 gm, were used for the experimental animals. The rats were fed. on the standard diet(st), protein free diet(pf) and imbalanced protein diet(ib) for twelve weeks respectively.
Hemoglobin, packed cell volume in blood, and total nitrogen, amino acid nitrogen, urea-nitrogen,. creatinine, transaminases(GPT, GOT) in liver and serum, and total nitrogen in small intestine, and: total nitrogen, urea-nitrogen in small intestine, and total nitrogen, urea-nitrogen, creatinine_ urea-nitrogen/creatinine ratio in urine were measured.
The results obtained are as follows;
1. The gained body weight were lower in pf group and ib group than those of st group.
The gained body weight fed for 12 weeks, were 80,0 lower in pf group than those of st group;. and the body weight of pf group for 5075 days feeding were 40^-60,o decreased, compared with the-stating weight, and then all of them died.
2. The change of the brain, liver, kidney, spleen and small intestine by feeding on imbalanced diet-for 12 weeks were no remarkable difference with the starting weight, but those of protein free diet-group were half or more decrease and those were significantly lower in spleen and small intestine-especially than the other organ
3. The contents of hemoglobin in pf group for 8 weeks feeding, and the packed cell volume in pf´ group for 8 weeks feeding and in ib group for 12 weeks feeding were decreased, but those of the-other feeding group were almost same value.
4. The total nitrogen in the liver, small intestine and serum of each diet group were no remarkable-difference respectively.
The contents of amino acid nitrogen in pf group for 2 and 6 weeks feeding were increased.
5. On transaminases;
a) The cycle of increase and decrease of GPT activities were come periodically and the interva´ of evcle were fast in the early stage of feeding and slow there-after.
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